Everything to know about the JIS Z 2801 Antimicrobial Efficacy Testing.

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New products are being developed in the textile industry every day. In addition to the traditional pattern, color, feel, and material differences, there are also innovations in the functional properties of products.

Today, products are produced to meet the different needs of people in addition to their clothing and protection functions. The characteristics of products belonging to various sectors such as health, safety, cosmetics, and computer science are now being added to textile products, and textile products are enabled to obtain new and different functions without touching the characteristics of appearance, touch, and comfort.

In this framework, antimicrobial products are widely used in daily life, especially in health institutions, hospitals, and hygienic purposes. Textile products used in various fields, such as baby clothes, upholstery, towels, napkins, tea towels, sportswear, and hotel textiles, are now produced with antimicrobial properties.

The JIS Z 2801 standard, published by the Japan Standards Association, provides a test method to determine the ability of plastics, metals, ceramics, and other antimicrobial surfaces to inhibit or kill the growth of microorganisms.

What is jis z 2801 test method? 

This jis z 2801 test method is highly sensitive to antimicrobial activity. It can be applied anywhere from healthcare facilities, hospitals, and clinics to residential buildings with a material capable of producing bacteria. This test method is designed to test the ability of hard surfaces to inhibit or kill microorganisms over a 24 hour contact period.

The standard has also become an industry standard for the antimicrobial performance of hard surfaces in the US. This standard has been published by the International Organization for Standardization (ISO) as ISO 22196. 

This jis z 2801 test method standard provides an excellent method for determining the level of antimicrobial activity of an antimicrobial surface. Among the various tests for surface antimicrobial activity, this test method is considered one industry standard.

The test organisms used were two E. coli strains (ATTC 25922 / ATTC 8739) and two Staphylococcus strains (ATTC 6538 / ATTC 6538p) grew at 37 ° C for 24 hours and had a working concentration of 5 x 105 cells. Dilute to ml.

  • The control and test surfaces are inoculated with working culture and covered with a sterile film that ensures uniform and intimate surface contact.
  • At baseline (t = 0), bacterial viability is determined by serial dilution and plating on appropriate agar while incubating treated and untreated samples at 37 ° C for 18 hours.
  • Clean the sample to remove bacteria from the surface. This extract is diluted stepwise and plated on the appropriate agar to count colony forming units.
  • The control verifies the dissolution method to ensure effective neutralization of the antibacterial agent.
  • The effectiveness of antibacterial compounds is determined by calculating the logarithmic reduction in bacterial viability caused by the test sample.